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Image Search Results
Journal: The EMBO Journal
Article Title: Transcriptional and chromatin profiling of human blood innate lymphoid cell subsets sheds light on HIV ‐1 pathogenesis
doi: 10.15252/embj.2023114153
Figure Lengend Snippet: A CD127 + and CD127 − cells from Lin − CD56 − population after gating on lymphoid, singlet, live, CD45 + cells of PBMCs. Lineage (Lin) markers include antibodies against: CD3, CD4, TCRαβ, TCRγδ, CD19, CD20, CD22, CD34, FcεRIα, CD11c, CD303, CD123, CD1a, and CD14. B Heatmap of differentially expressed genes by RNA‐Seq, sorted Lin − CD56 − CD16 − CD127 + versus Lin − CD56 − CD16 − CD127 − cells, from PBMCs of five donors (log2 fold change > 1, P adj < 0.01 determined by DESeq2, the postsort profiles are shown in Appendix Fig ). C Normalized counts of Lin − CD56 − CD127 + (red) and Lin − CD56 − CD127 − (blue) cell‐related genes from (B) by DESeq2 ( n = 5). D, E Reactome analysis based on enriched transcripts of Lin − CD56 − CD127 + cells (D) or Lin − CD56 − CD127 − cells (E). F Gating of Lin − CD56 hi NK, Lin − CD56 dim NK, Lin − CD56 − NK cells, and Lin − CD56 − CD16 − CD127 + ILCs. G The indicated populations in (F) were detected with isotype controls or antibodies against TBX21, CRTH2, and RORγT. Data information: All data were generated using blood from healthy HIV‐1‐negative donors.
Article Snippet: UCB was processed as previously described and underwent
Techniques: RNA Sequencing, Generated
Journal: The EMBO Journal
Article Title: Transcriptional and chromatin profiling of human blood innate lymphoid cell subsets sheds light on HIV ‐1 pathogenesis
doi: 10.15252/embj.2023114153
Figure Lengend Snippet:
Article Snippet: UCB was processed as previously described and underwent
Techniques: Control, Sequencing, Cell Stimulation, Multiplex Assay, Purification, Cell Isolation, Software